Journal: iScience
Article Title: Selective activation of PPARα maintains thermogenic capacity of beige adipocytes
doi: 10.1016/j.isci.2023.107143
Figure Lengend Snippet: Pemafibrate treatment in DIO mice after the induction of beige fat in thermoneutral conditions (A) Schematic illustration of experiments. WT B6/J male mice were acclimated to thermoneutrality (TN, 30°C) for 1 week, treated with CL316,243 (1 mg kg −1 ) for 1 week, and switched to pemafibrate (Pema group; 1 mg kg −1 ) or a vehicle (Off group) for 4 weeks with high-fat diet. The group treated with only the vehicle under the same conditions during the experiment was used as the control (Vehicle). (B) Body weight change of each group of mice. Body weight was measured once a week (n = 8–10 per group; mean ± SD). (C) Plasma glucose levels following a glucose tolerance test (IPGTT, 1 g kg −1 ) in each group of mice on week 12 (n = 8–10 per group; mean ± SEM). (D) Hematoxylin & Eosin and UCP1 immunohistochemical staining in iWAT of control, Off, and Pema group mice. Scale bar, 100 μm. (E) Relative mRNA levels of thermogenic genes in iWAT (n = 7–10 per group; mean ± SEM). (F) Western blot analysis of UCP1 protein levels. (G) Oxygen consumption rates in ex vivo inguinal adipose tissue in CL-Off or CL-Pema (1.0 mg/kg) mice. (H) Relative Fgf21 mRNA levels in the liver (n = 7–10 per group; mean ± SEM). (I) Plasma Fgf21 concentrations after vehicle or pemafibrate administration for 1 week in a beige adipocyte-induced mouse model (n = 4–5 per group; mean ± SEM). (B and C) Vehicle vs. CL-Pema (∗), CL-Off vs. CL-Pema ( † ), vehicle vs. CL-Off ( # ).
Article Snippet: Plasma FGF21 levels were analyzed using an ELISA kit (MF2100; R&D Systems, Minneapolis, MN, USA) ( I).
Techniques: Control, Immunohistochemical staining, Staining, Western Blot, Ex Vivo